Plasma metabolism of apolipoprotein A-IV in humans.

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Metabolism of apolipoprotein A-IV.

ApoA-IV was first described by Swaney, Reese, and Mer (1) as a component of rat HDL. Its presence has since been confirmed in dogs (2) as well as in man (3, 4). Apart from plasma, apoA-IV can also be demonstrated in human and rat mesenteric lymph (2, 5, 6), dog peripheral lymph (7), rat renal lymph (8), and rat bile (9). Despite the knowledge of apoA-IV's existence for over 10 years, relatively...

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Dynamics of apolipoprotein E metabolism in humans.

The dynamics of human apoE metabolism were explored by examining the effects of alimentary lipemia and postheparin lipolysis on the plasma level and lipoprotein distribution of apoE. In the studies of alimentary lipemia, fasting and postprandial plasma samples were obtained from five normal adult males, each of whom drank 100 g of corn oil. Although no change in the plasma concentration of apoE...

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Apolipoprotein A-IV: a protein intimately involved in metabolism.

The purpose of this review is to summarize our current understanding of the physiological roles of apoA-IV in metabolism, and to underscore the potential for apoA-IV to be a focus for new therapies aimed at the treatment of diabetes and obesity-related disorders. ApoA-IV is primarily synthesized by the small intestine, attached to chylomicrons by enterocytes, and secreted into intestinal lymph ...

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Distribution of apolipoprotein A-IV in human plasma.

Human apoA-IV was purified from delipidated urinary chylomicrons. Monospecific antibodies were raised in rabbits and used to develop a double antibody radioimmunoassay (RIA). Displacement of 125I-labeled apoA-IV by plasma or purified chylomicron apoA-IV resulted in parallel displacement curves, indicating that apoA-IV from both sources share common antigenic determinants. The apoA-IV level in p...

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Quantification of apolipoprotein A-IV in human plasma by immunonephelometry.

We developed a nephelometric procedure for determining concentrations of human plasma apolipoprotein (apo) A-IV. Results obtained correlate well with those measured by an established electroimmunodiffusion assay (r = 0.98; n = 56). Intra- and interassay CVs were 2.4% and 2.0%, respectively, indicating excellent precision and reproducibility. Various conditions of sample treatment, such as adequ...

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ژورنال

عنوان ژورنال: Journal of Lipid Research

سال: 1988

ISSN: 0022-2275

DOI: 10.1016/s0022-2275(20)38785-x